THE AMINO ACID COMPOSITION OF ANTERIOR HYPOPHYSEAL GROWTH HORMONE
نویسندگان
چکیده
منابع مشابه
The Amino Acid Composition of Human Pituitary Growth Hormone
The amino acid content in human hypophyseal growth hormone has been determined by chromatography on resin columns. On the basis of 29,000 for the molecular weight, the empirical formula of the hormone was obtained: lys(13)his(5)arg(14)asp(27) thr(14)ser(23)glu(34)pro(12)gly(13)ala(12)-(1/2 cys)(6)val(12)met(4)-ileu(10)leu(31)tyr(10)phe(15)try(1)(NH(3))(32).
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Sedimentation velocity was measured in a Spinco ultracentrifuge’ operating at 165,000 X g. The hormone solutions (0.5 per cent) were prepared with borate buffer of pH 9.7 or saline at pH 9.1. Table I presents a typical run in a borate buffer; the sedimentation velocity constant was calculated by the procedure described by Svedberg and Pedersen (3). It may be noted that the individual values of ...
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many formulae and figures. Its many chapter subheadings give the book its rather terse, outline form. A discussion of methods of isolation, characterization, and determination of purity is followed by successive chapters dealing with reaction rates as a function of substrate concentration, inhibitor action, temperature, and pH. Thermodynamic concepts are well developed in chapters on energetics...
متن کاملSedimentation behavior of hypophyseal growth hormone.
In a previous publication, it was reported that solution of the hypophyseal growth hormone in acetate buffers results in an increment of its intrinsic viscosity with an accompanying increase of molecular weight (1). Later, Smith et al. (2) confirmed these observations in ultracentrifugal studies. The purpose of this present investigation has been to study the sedimentation behavior of the hormo...
متن کاملZone electrophoresis of hypophyseal growth hormone (somatotropin) on starch.
Growth hormone preparations were obtained by methods previously described (1, 3) from the anterior lobes of ox pituitary glands. The biological activity of these preparations was estimated by the tibia test (5) in hypophysectomized rats. Electrophoretic experiments were carried out in a cold room at 3”. The technique of zone electrophoresis is a modification of the method of Kunkel and Slater (...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1947
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)30868-2